Fluorescence　imaging　holds　great　potential　as　a　powerful　diagnostic　tool　for　tumor　cell　visualization.　However,　a　significant　challenge　in　fluorescence　imaging　is　the　high　background　signal,　which　obscures　the　tumor-specific　signal　and　reduced　the　signal-to-noise　ratio　(SNR)　of　imaging,　thereby　reducing　the　accuracy　of　tumor　detection　and　delineation.　In　this　research,　we　designed　and　synthesized　an　amphiphilic　disulfide　dicyanine　ss-diCy7,　which　can　self-assemble　into　nanoparticles　with　uniform　dispersion　in　aqueous　environments.　The　fluorescence　intensity　of　these　nanoparticles　is　significantly　reduced　by　96%　due　to　aggregation-induced　quenching　arising　from　π-π　stacking.　The　nanoparticles　exhibit　a　highly　specific　response　to　glutathione　(GSH)　in　vitro,　resulting　in　a　substantial　enhancement　of　fluorescence　intensity　by　a　24-fold.　The　enhancement　was　also　achieved　in　cell　and　mouse　imaging　experiments.　In　addition,　in　the　mouse　tumor　model,　ss-diCy7　nanoparticles　demonstrated　superior　performance　compared　to　traditional　mono-cyanine　dyes,　offering　a　reduced　background　signal　and　prolonged　fluorescence　duration.　This　work　is　anticipated　to　contribute　to　the　high-resolution　tumor　imaging.　©　2025　Elsevier　B.V.