Striped　patterns　have　broad　applications　in　recognition,　cell　culture,　and　droplet　manipulation.　However,　a　rapid　and　robust　assembly　method　for　various　striped　patterns　and　their　application　demonstrations　are　still　lacking.　Here,　we　develop　a　robust　and　universal　confined-area　assembly　method　to　position　various　building　blocks　into　striped　patterns　using　an　accelerated　evaporation　strategy　and　demonstrate　the　applications　of　assembled　patterns　in　molecular　pattern　and　cell　culture.　The　lowered　pressure　or　heat　is　used　to　accelerate　solvent　evaporation,　promoting　fast　convection　flow　in　liquid　and　inducing　stick-slip　motion　of　triple-phase　contact　line.　Microgel,　nanoparticles,　high-density　microparticles,　bacteria,　and　solutes　can　form　striped　patterns　using　the　proposed　method,　showing　its　robust　for　fabricating　various　patterns.　The　wettability,　encapsulating　property　and　cell　guidance　ability　of　striped　patterns　are　investigated,　exhibiting　their　potential　applications　in　different　fields.　The　proposed　accelerated　evaporation-induced　confined-area　assembly　is　a　rapid　and　effective　approach　for　preparing　striped　patterns　from　different　components　for　advanced　functions.