Supported　catalysts　have　gradually　become　a　research　hotspot　in　the　field　of　removing　antibiotics　from　wastewater.　In　this　study,　CuFe2O4@2D-V　composite　catalyst　was　prepared　by　basic　hydrothermal　synthesis.　Twodimensional　expanded　vermiculite　(2D-V)　was　prepared　by　high　temperature　and　ultrasonic　stripping　to　provide　a　loading　platform　for　CuFe2O4　metal　ions.　The　characterization　of　CuFe2O4@2D-V　was　analysis　by　SEM,　XRD　and　EDS.　Then,　levofloxacin　(LVX)　was　chosen　as　representative　to　investigate　the　catalytic　performance　of　CuFe2O4@2D-V　by　persulfate　(PMS)　activation.　The　optimal　preparation　conditions　of　CuFe2O4@2D-V　and　operating　parameters　of　activating　PMS　to　remove　LVX　were　also　studied.　The　result　showed　that　under　the　condition　of　CuFe2O4　loading　50　%,　initial　pH　9.2,　LVX　concentration　10　mg/L,　catalyst　concentration　0.5　g/L　and　PMS　concentration　of　0.3　mM,　the　removal　efficiency　and　reaction　rate　of　LVX　could　reach　89　%　and　0.071　min-1,　respectively.　Meanwhile,　the　catalyst　had　a　good　stability　and　high　reusability.　Based　on　the　analysis　of　intermediate　products　by　LC-MS,　three　possible　degradation　pathways　were　proposed.　Quenching　experiments　found　that　non-radical　1O2　dominated　LVX　degradation　process.　Also,　the　redox　reaction　between　Cu　(I)/Cu　(II)　and　Fe　(II)/Fe　(III)　played　an　important　role　in　LVX　removal　process.　This　work　might　fill　the　gap　in　the　application　of　vermiculite　as　a　two-dimensional　carrier,　and　provide　further　understanding　for　antibiotic　wastewater　treatment　by　metal　based　two-dimensional　composite　materials.