The　novel　isolated　strain　JQ-H3　exhibited　heterotrophic　nitrification-aerobic　denitrification　(HN-AD)　ability　using　poly　(e-caprolactone)　(PCL)　as　a　sole　carbon　source　under　aerobic　conditions.　This　was　the　first　time　that　a　PCL-degrading　bacterium　was　characterized　to　be　with　capability　of　nitrifying　and　denitrifying　performances.　Strain　JQ-H3　removed　approximately　95.40%　of　NH4+-N　(102.40　mg/L),　about　91.1%　of　NO3-　-N　(99.01　mg/L),　with　the　maximum　specific　removal　rates　of　0.308　mg　NH4+-N/mg　DCW/h　and　0.220　mg　NO3-　-N/mg　DCW/h,　respectively.　The　functional　genes　amoA,　napA,　nirS,　cnorB,　and　nosZ　were　successfully　expressed　during　the　nitrogen　removal　process.　An　increasing　PCL　concentrations　caused　a　decline　in　cell　growth　and　nitrogen　removal　efficiency,　which　was　attributed　to　the　decrease　in　the　rate　of　the　PCL　hydrolysis　and　the　amount　of　dissolved　organic　carbon　(DOC)　release.　These　results　demonstrated　the　strain　as　an　ideal　candidate　for　nitrogen　removal　from　wastewater　by　using　PCL　as　carbon　source.