Digital　holography　is　proposed　as　a　non-destructive　fingerprint　acquisition　method　in　the　criminal　investigation　field.　Digital　holography　is　a　new　imaging　technology　which　combines　the　advantages　of　both　the　optical　holography　and　digital　processing.　As　an　advanced　optical　diagnostic　tool,　digital　holography　allows　to　provide　the　quantitative　phase　information　and　the　intensity　information　simultaneously.　Based　on　the　off-axis　digital　Fresnel　holography,　the　principle　of　recording　and　reconstruction　are　analyzed,　the　reconstruction　methods　of　the　fingerprint　amplitude　and　phase　image　are　investigated,　and　the　experimental　results　are　presented.　Both　the　theoretical　analysis　and　the　experimental　results　showed　that　digital　holography　does　not　destroy　the　on-site　fingerprints,　overcomes　the　shortcomings　of　traditional　fingerprint　acquisition　methods,　and　as　a　simple　and　effective　acquisition　method　can　be　applied　to　the　criminal　investigation　field.　©　2011　IEEE.