Mesoporous　TiO2　structure　(mp-TiO2)　with　a　compact　TiO2　blocking　layer　(bl-TiO2)　has　been　recognized　as　an　effective　photoanode　for　high-performance　dye-sensitized　solar　cells　(DSSCs).　However,　producing　both　mesoporous　and　compact　TiO2　layers　by　conventional　method　requires　a　high-temperature　furnace　process　via　a　two-step　manufacturing　route.　Here,　a　one-step　laser　technique　has　been　successfully　developed　to　generate　both　mesoporous　and　compact　TiO2　layers　on　tin-doped　indium　oxide　(ITO)　coated　glass　by　a　millisecond　pulsed　fiber　laser　with　a　wavelength　of　1070　nm　in　ambient　atmosphere.　Compared　with　the　conventional　furnace　process,　the　laser　process　exhibits　a　significant　reduction　of　overall　fabrication　time　from　5　h　20　min　to　2　min.　More　importantly,　the　DSSCs　fabricated　by　the　laser　process　show　a　remarkable　improvement　with　the　maximum　power　conversion　efficiency　(PCE)　by　34%　compared　with　the　DSSCs　fabricated　by　the　furnace.　The　short-circuit　current　density　(J(sc))　reaches　16.5　mA　cm(-2)　for　the　DSSCs　fabricated　by　the　laser　process　while　10.6　mA　cm(-2)　is　obtained　for　those　fabricated　by　the　furnace　process.　The　improvement　is　associated　with　the　increased　dye　adsorption,　decreased　charge　transfer　resistance,　and　increased　electron　lifetime　of　the　TiO2　photoanode　fabricated　by　the　laser　process.