Purpose:　We　aimed　to　investigate　RF-EMR-induced　cell　malignant　transformation.　Methods:　We　divided　Balb/c-3T3　cells　into　sham　and　expo　groups.　The　expo　groups　were　exposed　to　a　1800　MHz　RF　continuous　wave　for　40　and　60　days,　for　4　h　per　day.　The　sham　group　was　sham-exposed.　Cells　were　harvested　for　a　cell　transformation　assay,　transplantation　in　severe　combined　immune　deficient　(SCID)　mice,　soft　agar　clone　formation　detection,　and　a　transwell　assay.　The　mRNA　microarray　assay　was　used　to　declare　key　genes　and　pathways.　Results:　The　exposed　Balb/c-3T3　cells　showed　a　strong　increase　in　cell　proliferation　and　migration.　Malignant　transformation　was　observed　in　expo　Balb/c-3T3　cells　exposed　for　40　days　and　60　days,　which　was　symbolized　with　visible　foci　and　clone　formation.　Expo　Balb/c-3T3　cells　that　were　exposed　for　40　days　and　60　days　produced　visible　tumors　in　the　SCID　mice.　Lipid　metabolism　was　the　key　biological　process　and　pathway　involved.　The　mevalonate　(MVA)　pathway　was　the　key　metabolic　pathway.　The　interacted　miRNAs　could　be　further　research　targets　to　examine　the　molecular　mechanism　of　the　carcinogenic　effects　of　long-term　exposure.　Conclusion:　Exposure　for　40　and　60　days　to　1800　MHz　RF-EMR　induced　malignant　transformation　in　Balb/c-3T3　cells　at　the　SAR　of　8.0　W/kg.　We　declared　that　lipid　metabolism　was　the　pivotal　biological　process　and　pathway.　The　MVA　pathway　was　the　key　metabolic　pathway.