Hydrogen　sulfide　(H2S)　is　a　gaseous　signaling　molecule　that　plays　an　important　role　in　regulating　various　physiological　activities　in　biological　systems.　As　the　fundamental　structural　and　functional　unit　of　organisms,　cells　are　closely　related　to　the　homeostasis　of　their　internal　environment.　The　levels　of　H2S　in　different　organelles　maintain　a　certain　balance,　and　any　disruption　of　this　balance　will　lead　to　various　functional　abnormalities　that　affect　the　health　of　organisms.　Fluorescent　imaging　technology　provides　unique　merits,　such　as　simplicity,　non-invasiveness,　and　real-time　monitoring,　and　has　become　a　powerful　approach　for　the　detection　of　molecules　in　biological　systems.　Based　on　the　special　physicochemical　properties　of　H2S,　numerous　H2S-specific　fluorogenic　probes　have　been　designed　with　different　recognition　mechanisms　that　enable　rapid　and　accurate　detection　of　H2S　in　cells.　Therefore,　this　review　briefly　illustrates　the　design　strategies,　response　principles,　and　biological　applications　of　H2S-specific　fluorescent　probes　and　aims　to　provide　relevant　researchers　with　insight　for　future　research.