The　novel　ratiometric　fluorescent　probe　HPQRB　with　an　ESIPT　effect　based　on　Michael　addition　for　highly　sensitive　and　fast　detection　of　sulfite　in　living　HepG2　cells　is　reported.　HPQRB　can　be　easily　synthesized　by　a　two-step　condensation　reaction.　HPQRB　has　a　large　emission　shift　(Delta　lambda=116　nm),　which　is　beneficial　for　fluorescence　imaging　research,　and　its　sulfite-responsive　site　is　based　on　a　rhodamine-like　structure　with　the　emission　peak　at　566　nm,　which　decreases　with　increasing　sulfite　concentration.　and　its　HPQ　structure　always　has　an　ESIPT　effect　throughout　the　reaction　process,　keeping　the　emission　peak　at　450　nm　as　a　self-reference.　In　particular,　HPQRB　has　high　selectivity　for　sulfite　and　responds　quickly　(within　30　s)　with　a　low　detection　limit　(44　nM).　Furthermore,　HPQRB　has　been　successfully　used　for　fluorescence　imaging　of　sulfite　in　HepG2　cells,　demonstrating　the　superior　ability　to　detect　sulfite　under　physiological　conditions.