Differential　distribution　of　neonicotinoids　(NEOs)　in　albumen,　yolk,　and　eggshell　is　a　critical　factor　influencing　their　bio-accumulative　behavior　and　the　subsequent　human　health　risks.　However,　there　is　currently　no　relevant　research　available.　We　collected　62　egg　samples　from　31　sampling　sites　across　China　and　analyzed　the　concentrations　and　characteristics　of　12　parents　NEOs　(p-NEOs)　and　8　metabolites　NEOs　(m-NEOs)　in　albumen,　yolk,　and　eggshell.　NEOs　were　frequently　detected　in　differentiated　egg　matrices,　with　the　highest　concentrations　observed　in　Northeast　China.　The　concentrations　of　m-NEOs　were　generally　higher　than　those　of　p-NEOs.　A　positive　correlation　was　found　between　the　distribution　of　m-NEOs　in　albumen　and　their　logKow　(p　＜　0.05).　Dietary　intake　exposure　posed　a　higher　risk　of　NEOs　to　children　and　females.　The　toxicity　equivalence　(TEQ)　of　5hydroxy-imidacloprid　and　thiacloprid-amide,　in　interaction　with　androgen　and　estrogen　receptors,　was　higher　compared　to　other　NEOs.　Network　toxicology　and　molecular　docking　indicated　that　AKT1　may　serve　as　the　core　target　for　reproductive　toxicity　induced　by　dinotefuran,　cycloxaprid,　and　nitenpyram　exposure.　This　study　provided　valuable　data　on　the　occurrence,　differential　distribution,　and　reproductive　exposure　risk　of　NEOs　in　eggs　for　the　first　time.　These　findings　are　instrumental　for　future　management　policies　concerning　the　environmental　behavior　and　health　effects　of　NEOs.