Surface-enhanced　Raman　spectroscopy　(SERS)　is　an　ultra-sensitive　analytical　tool　that　can　effectively　detect　and　identify　molecules　by　their　unique　vibrational　fingerprints.　Development　of　SERS　substrates　with　good　stability,　high　sensitivity　and　reproducibility　is　still　a　big　challenge　in　practical　applications.　Recently,　2D　materials/metallic　hybrid　SERS　substrates　provide　a　new　prospect　to　improve　the　SERS　performance.　Here,　we　obtain　a　monolayer　MoS2　covered　silver　nanoparticle　(AgNP)　array　as　a　high-performance　SERS　substrate.　Annealing　temperature-dependent　SERS　signals　on　the　hybrid　substrate　have　been　explored.　The　optimum　SERS　performance　was　obtained　at　290　t　(the　detection　limit　of　10(13)　M　for　Rhodamine　6G　and　the　corresponding　SERS　enhancement　factor　of　8.3　x　10(9)),　which　is　attributed　to　the　better　contact　between　AgNPs　and　MoS2　and　the　uniform　AgNPs　with　appropriate　particle　sizes.　The　prepared　MoS2/AgNPs　hybrid　substrates　also　have　been　utilized　to　detect　various　molecules,　which　demonstrates　a　great　potential　for　applications　in　food　safety　and　biochemical　environmental　detection.　(C)　2022　Elsevier　B.V.　All　rights　reserved.