The　impact　of　temperature　on　the　biological　removal　of　hydrogen　sulfide　(H2S)　from　air　is　critical　to　its　effective　ap-plication　in　cold　regions　or　seasons.　This　study　investigated　the　effect　of　seasonal　temperature　variations　(7-30　degrees　C)　on　the　H2S　removal　performance　of　a　biotrickling　filter　system,　with　an　effective　H2S　elimination　capacity　of　98.1　g/m3/h　(removal　efficiency　=　83.1　%)　achieved　at　temperatures　of　10-12　degrees　C.　Biofilm　growth　was　found　to　be　accelerated　by　increased　secretion　of　extracellular　polymeric　substances,　enhanced　biofilm　adhesion　capacity　and　relatively　high　levels　of　elemental　sulfur　accumulation,　which　help　to　retain　heat　within　the　filter　bed　under　cold　conditions.　High-throughput　sequencing　showed　that　the　psychrotolerant　sulfur-oxidizing　bacterium　(SOB)　Metallibacterium　was　gradually　enriched　(54.8　%)　at　temperatures　below　15　degrees　C.　The　major　pathways　of　sulfur　metab-olism　under　low　temperature　conditions　were　determined　based　on　the　detection　of　enzymes　related　to　sulfur　metab-olism.　Finally,　a　strategy　to　enrich　Metallibacterium　was　proposed　to　promote　the　application　of　biodesulfurization　under　low　temperature　conditions.