The　cooperative　vehicle-infrastructure　system　(CVIS)　offers　opportunities　to　further　enhance　the　potential　of　energy　saving　and　emission　reduction　for　eco-driving.　However,　it　is　still　not　clear　about　the　effectiveness　of　CVIS　on　promoting　eco-driving　nor　the　multivariate　effectiveness　of　CVIS　designed　for　eco-driving　on　traffic　flow　and　safety　characteristics.　A　dedicated　ecological　lane　(Ecolane)　and　ecological　human-machine-interaction　(EcoHMI)　based　CVIS　(Ecolane-HMI-CVIS)　were　developed　based　on　driving　simulator　technology.　An　experiment　of　35　participants　was　conducted　to　study　eco-driving　behavior　and　final　utility　to　identify　the　influence　of　Ecolane-HMI-CVIS.　The　results　indicated　that　the　Ecolane-HMI-CVIS　enhanced　eco-driving　behavior　and　reduced　emission,　with　CO　and　NOx　significantly　reduced　by　10.72　%　and　9.83　%　respectively.　The　Ecolane-HMI-CVIS　reduced　the　headway　and　promoted　vehicle　operation　stability,　ordering,　and　improved　traffic　capacity　about　10　%.　No　negative　impact　of　Ecolane-HMI-CVIS　was　observed　on　traffic　safety.　This　study　developed　a　test　platform　based　on　driving　simulator　to　explore　the　multivariate　influence　of　Ecolane-HMI-CVIS.　In　addition　to　analyzing　the　overall　effect　of　Ecolane-HMI-CVIS　during　the　whole　experimental　section,　its　detailed　influence　at　each　key　zone　and　in　spatial　change　process　were　also　analyzed.　This　research　contributed　to　better　understanding　of　the　working　mechanism　and　effectiveness　of　Ecolane-HMI-CVIS,　and　provide　technical　and　policy　references　of　CVIS　based　eco-driving　for　traffic　management　departments.　©　2023,　KSAE.