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Author：

Wang, Q. (Wang, Q..) | Wang, G. (Wang, G..) | Tang, D. (Tang, D..) | Liu, Z. (Liu, Z..) | Hong, Q. (Hong, Q..) | Zhang, Y. (Zhang, Y..) | Zong, Q. (Zong, Q..) | Hou, X. (Hou, X..) | Yao, M. (Yao, M..) | Zheng, Y. (Zheng, Y..) | Sheng, C. (Sheng, C..) | Zhou, Z. (Zhou, Z..)

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Scopus

Abstract：

Microplastics　（MPs）　exist　in　the　natural　environment　for　a　long　time，and　can　endanger　human　health　through　respiration　and　food　intake.　Microplastics　not　only　have　biological　toxicity，but　also　can　adsorb　various　components　to　form　complex　pollutants.　To　explore　the　adsorption　characteristics　of　microplastics　on　plasmid　DNA　and　the　effect　of　plasmid　transfection　mediated　by　microplastics.　In　this　study，the　adsorption　of　polystyrene　microplastics　（PS-MPs）　on　plasmid　DNA　（pcDNA　3.1-EGFP）　was　characterized　by　agarose　gel　electrophoresis　and　transmission　electron　microscopy.　After　the　microplastics　and　its　plasmid　DNA　adsorption　complex　were　transfected　into　human　esophageal　epithelial　cells　EC109，RT-qPCR　was　used　to　detect　the　intracellular　transcription　of　eGFP　gene　and　neomycin　resistance　gene　（neoR）　derived　from　plasmid　pcDNA　3.1-EGFP　in　EC109　cells.　Moreover，the　expression　and　cell　distribution　of　green　fluorescent　protein　derived　from　plasmid　pcDNA　3.1-EGFP　were　observed　under　confocal　microscope.　The　toxic　effects　of　microplastics　and　their　plasmid　DNA　adsorption　observed　under　confocal　microscope.　The　toxic　effects　of　microplastics　and　their　plasmid　DNA　adsorption　complexes　on　EC109　cells　were　examined　by　microscope　and　CCK-8　test.　The　results　showed　that　polystyrene　microplastics　could　effectively　adsorb　plasmid　DNA，and　the　adsorption　effect　of　polystyrene　microplastics　on　plasmid　DNA　was　negatively　correlated　with　the　particle　size　of　microplastics，and　positively　correlated　with　the　concentration　of　microplastics.　The　equilibrium　time　of　microplastics　adsorption　on　plasmid　DNA　is　about　12　hours.　Polystyrene　microplastics-plasmid　DNA　complex　showed　a　cluster　phenomenon.　The　effective　transcription　of　eGFP　gene　and　resistance　gene　（neoR）　and　the　expression　of　green　fluorescent　protein　were　detected　in　the　transfected　EC109　cells，and　there　was　no　significant　change　in　cell　morphology　and　activity.　This　study　shows　that　polystyrene　microplastics　can　effectively　adsorb　plasmid　DNA　and　mediate　the　transfection　of　plasmid　DNA　into　eukaryotic　cells.　©　2023　Science　Press.　All　rights　reserved.

Keyword：

toxicity polystyrene microplastics human esophageal cancer cells gene transfer plasmid DNA adsorption

Author Community：

  • [ 1 ] [Wang Q.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 2 ] [Wang G.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 3 ] [Wang G.]Department of Laboratory Medicine, China-Japan Friendship Hospital, Beijing, 100029, China
  • [ 4 ] [Tang D.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 5 ] [Liu Z.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 6 ] [Hong Q.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 7 ] [Zhang Y.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 8 ] [Zong Q.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 9 ] [Hou X.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 10 ] [Yao M.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 11 ] [Zheng Y.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 12 ] [Sheng C.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China
  • [ 13 ] [Zhou Z.]Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, China

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Source ：

Acta Scientiae Circumstantiae

ISSN： 0253-2468

Year： 2023

Issue： 7

Volume： 43

Page： 383-390

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 12

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