Ferroptosis,　a　commonly　observed　type　of　programmed　cell　death　caused　by　abnormal　metabolic　and　biochemical　mechanisms,　is　frequently　triggered　by　cellular　stress.　The　occurrence　of　ferroptosis　is　predominantly　linked　to　pathophysiological　conditions　due　to　the　substantial　impact　of　various　metabolic　pathways,　including　fatty　acid　metabolism　and　iron　regulation,　on　cellular　reactions　to　lipid　peroxidation　and　ferroptosis.　This　mode　of　cell　death　serves　as　a　fundamental　factor　in　the　development　of　numerous　diseases,　thereby　presenting　a　range　of　therapeutic　targets.　Single-cell　sequencing　technology　provides　insights　into　the　cellular　and　molecular　characteristics　of　individual　cells,　as　opposed　to　bulk　sequencing,　which　provides　data　in　a　more　generalized　manner.　Single-cell　sequencing　has　found　extensive　application　in　the　field　of　cancer　research.　This　paper　reviews　the　progress　made　in　ferroptosis-associated　cancer　research　using　single-cell　sequencing,　including　ferroptosis-associated　pathways,　immune　checkpoints,　biomarkers,　and　the　identification　of　cell　clusters　associated　with　ferroptosis　in　tumors.　In　general,　the　utilization　of　single-cell　sequencing　technology　has　the　potential　to　contribute　significantly　to　the　investigation　of　the　mechanistic　regulatory　pathways　linked　to　ferroptosis.　Moreover,　it　can　shed　light　on　the　intricate　connection　between　ferroptosis　and　cancer.　This　technology　holds　great　promise　in　advancing　tumor-wide　diagnosis,　targeted　therapy,　and　prognosis　prediction.　Graphical　Abstract