Laser　powder　bed　fusion　(LPBF)　fabricated　Al-Mn-Mg-Sc-Zr　alloy　generally　possesses　a　bi-modal　microstructure　of　columnar　grains　and　equiaxed　grains.　Such　an　anisotropic　microstructure　would　introduce　varied　tensile　performance　in　different　orientations.　To　date,　few　study　on　the　microstructure　and　tensile　property　anisotropy　have　been　reported　for　LPBF　fabricated　Al-Mn-Mg-Sc-Zr　alloys　built　at　different　layer　thicknesses,　which　limits　the　exploration　in　mechanical　property　optimization.　In　this　work,　the　microstructure　anisotropy　and　room-　temperature　tensile　properties　of　LPBF　produced　and　peak-aged　Al-Mn-Mg-Sc-Zr　alloys　built　at　30　and　60　mu　m　layer　thicknesses　were　systematically　investigated　by　scanning　electron　microscope,　transmission　electron　microscope,　and　tensile　testing.　A　higher　layer　thickness　of　60　mu　m　resulted　in　coarser　grains　with　the　precipitates　size　remained　similarly　compared　to　the　30　mu　m　specimens.　This　led　to　a　reduced　yield　strength　in　60　mu　m　specimens　(492-509　MPa)　comparing　to　those　in　30　mu　m　specimens　at　502　MPa-510　MPa.　In　addition,　the　existence　of　columnar　grains　within　melt　pools　contributed　to　a　larger　effective　slip　length　in　the　vertical　direction　than　that　in　the　horizontal　orientation.　Such　difference　in　effective　slip　length　in　the　loading　direction　contributed　to　a　lower　strength　in　vertical　orientations.　For　ductility,　the　slightly　higher　defect　level　in　60　mu　m　specimens　(0.58%)　resulted　in　reduced　elongations　of　3%-6%　compared　to　those　of　30　mu　m　specimens　(0.10%).　The　ductility　anisotropy　was　attributed　to　the　preferential　distribution　of　gas　pores　and　keyhole　defects　at　melt　pool　boundaries.