Microstructure　anisotropy　and　high-temperature　(100　degrees　C　-250　degrees　C)　tensile　property　were　investigated　for　laser　powder　bed　fusion　prepared　and　peak　aged　Al-Mn-Mg-Sc-Zr　alloys.　The　peak-aged　Al-Mn-Mg-Sc-Zr　alloy　had　a　bi-modal　microstructure　of　columnar　grain　with　melt　pool　and　equiaxed　grain　at　melt　pool　boundary.　The　yield　strength　and　ductility　decreased　with　increasing　tensile　temperatures.　The　decreased　yield　strength　which　was　attributed　to　the　decreased　thermal　activation　of　dislocations　and　Peierls-Nabarro　stress　at　high　temperatures.　The　reduced　ductility　was　attributed　to　the　increased　Al6(Mn,　Fe)　precipitates＇　size　and　the　preferential　crack　formation　in　Al6(Mn,　Fe)　at　grain　boundaries.　The　horizontal　specimens　had　higher　yield　strength　and　elongation　at　fracture　than　vertical　counterparts.　The　yield　strength　anisotropy　was　related　to　the　different　effective　slip　lengths　(grain　sizes)　of　columnar　grains　in　different　orientations,　and　the　ductility　anisotropy　was　caused　by　different　fraction　of　melt　pool　boundaries　on　horizontal　and　vertical　specimens.