Magnetic　separation(MS)　is　frequently　used　to　enrich　specific　targets　from　biological　samples,　which　is　commonly　performed　using　antibodies　coupled　immunomagnetic　beads　(IMBs).　However,　due　to　the　high　cost　of　antibodies　and　difficulties　in　the　process　of　releasing　captured　targets,　IMBs　have　limitations　for　large-scale　enrichment　of　targeted　bioproducts.　The　specificities　and　affinities　of　aptamers　towards　their　targets　are　in　line　with　antibodies,　but　with　characteristic　of　significantly　lower　costs　of　preparation,　simpler　structures,　and　higher　chemical　stability.　In　this　study,　taking　advantages　of　the　distinct　adsorption　features　of　graphene　for　single-stranded　and　double-stranded　nucleic　acids,　we　designed　a　novo　system　as　bioseparation　method　that　could　display　aptamers　on　graphene　surfaces　to　enrich　targets　and　then　manipulate　their　release.　This　system　mainly　includes　two　units.　Take　CD63　aptamer　as　an　example:　double-stranded　oligonucleotides　scaffold　attached　to　the　graphene　surface　for　CD63　aptamer　exhibition;　and　a　single-stranded　oligonucleotide　complementary　to　the　scaffold　bipod　sequence　to　desorb　the　captured　targets　from　graphene.　In　this　study,　firstly,　using　graphene　oxide　(GO)　paper　as　a　graphene　surface,　we　fluorescently　labeled　scaffolds　or　CD63　proteins　and　compared　the　fluorescence　intensity　difference　on　the　GO　paper　before　and　after　scaffold　or　CD63　protein　release.　Subsequently,　a　novo　material,　amino-modified　graphene-shelled　iron-nitrogen　magnetic　beads　were　introduced　to　carry　the　scaffold,　and　used　to　capture　CD63　proteins　from　cell　lysates.　The　results　indicate　that　this　scaffold　can　display　the　aptamer　on　the　graphene　surface　for　CD63　protein　capture　and　controlled　release.　Using　selected　modified　graphene　magnetic　beads　carried　with　the　scaffold,　we　achieved　the　capture　of　CD63　proteins　from　cell　lysates.　This　system　is　expected　to　enrich　various　proteins　such　as　CD63,　or　capture　exosomes　by　hooking　membrane　proteins.　©　2024　Chinese　Society　of　Biochemistry　and　Molecular　Biology,　Peking　University.　All　rights　reserved.