The　recovery　of　gold　(Au)　from　waste　printed　circuit　boards　(WPCBs)　derived　from　discarded　mobile　phones　is　crucial　for　the　resource　preservation　and　environmental　protection.　In　this　context,　thiosulfate　has　emerged　as　a　viable　alternative　to　the　toxic　compound　cyanide　for　Au　leaching.　However,　the　conventional　thiosulfate　leaching　system　has　a　main　problem　that　is　large　thiosulfate　consumption,　which　limits　its　commercial　application.　In　this　study,　we　developed　a　novel　thiosulfate　leaching　system　that　utilizes　a　cobalt　(Co(II))-glycine　(gly)　complex　as　the　catalyst.　Our　findings　demonstrate　that　the　Co(II)-glycine-thiosulfate　system　exhibits　excellent　Au　leaching　performance　coupled　with　low　thiosulfate　consumption.　The　effects　of　several　parameters　on　Au　leaching　efficiencies　and　thiosulfate　consumption　were　investigated.　Increasing　the　concentrations　of　Co(II),　thiosulfate,　or　glycine　was　beneficial　to　the　Au　leaching.　However,　increasing　temperature　promoted　the　oxidative　decomposition　of　thiosulfate　and　reduced　cobalt　stability,　negatively　impacting　Au　leaching.　Additionally,　small　amounts　of　base　metals　remained　after　pretreatment　of　WPCBs　using　HCl　system　and　H2SO4-H2O2　system.　Therefore,　the　roasting-assisted　leaching　process　eliminated　the　adverse　effects　of　these　impurity　metals　and　further　improved　Au　leaching　efficiency　to　97.8%　while　also　decreased　thiosulfate　consumption.　The　recovery　of　Au　from　the　pregnant　leach　solution　was　also　studied,　and　our　findings　demonstrated　that　N1923　can　efficiently　extract　Au　from　the　solution　through　anion　exchange.　Our　study　developed　a　novel　and　efficient　Au　thiosulfate　leaching　system　catalyzed　by　the　Co(gly)3/Co(S2O3)34−　redox　couple,　and　put　forward　a　promising　approach　to　enhance　Au　recyclability　from　WPCBs　while　significantly　reducing　thiosulfate　consumption.　©　2023